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Hitachi

Hitachi High-Technologies in Canada

Hitachi L-8900 amino acid analyzer

The L-8900 Amino Acid Analyzer is a dedicated system that continues the long line of Hitachi amino acid systems.

Overview

High Speed Amino Acid Analyzer from Hitachi

Hitachi has manufactured over 1800 Amino Acid Analyzers for over40 years. The L-8900 is capable of analyzing physiological fluids and protein hydrolysates. If you have special requirements, the L-8900 is the one to choose! Hitachi offers the best solution to amino acid analysis.

High Sensitivity: Reaction Column vs. Reaction Coil:

In conventional analyzers, sample zone spreading within the reaction coil causes adverse effects on separation. The L-8900 features a special reaction column (patent pending) to maintain good separation of the sample zone and yet offering high sensitivity and high-speed analysis.

Reaction Coil

Before reaction After reaction
The speed of the mobile phase is highest at the center of the tube, causing peak broadening.

Linear Velocity
By filling the reaction column with beads, the mobile phase moves less smoothly, resulting in a homogeneous flow without peak broadening.

Exceptionally Fast Analysis with Outstanding Detection Limits

Column before
Column before
Standard amino acid determination takes just 30 minutes (net) and physiological fluid determination can be carried out fully automatically in just 110 minutes.

Front Access

No space is needed at the side of the instrument for maintenance. Just open the front door to replace consumables, columns and the liquid waste container.

No Need for Refrigeration

The L-8900's unique post column delivery design mixes the two ninhydrin reagents just prior to amino acid derivatization. This means they can be stored for a year without refrigeration.

Specifications

Performance (Using Protein Hydrolysate Analysis - 30 min Standard Analysis Method)
Net Analysis Time 30 min
Reproducibility of Retention Time CV 0.3% (Arg), 0.5% (Ala)
Reproducibility of Peak Area CV 1.0% (Gly, His)
Detection Limit 3 pmol (S/N = 2, Asp)
Amino Acid Analyzer Main Unit
Column Size: 4.6 mm i.d. x 60 mm
(Resin: 3-µm Hitachi Customer Ion Exchange Resin)
Injection Pump Pressure: 0 - 20 Mpa (2900 psi)
Flow Rate: 0.00 - 0.99 mL/min
Autosampler
Injection Methods Direct injection method
Sample Vial Capacity 1.5 mL
Number of Sample Vials Accommodated 200 (200 when optional cooler installed)
Injection Volume 0.1 - 100 µL
Column Oven
Method Peltier
Temperature Setting 20 - 85°C (in increments of 1°C)
Reaction Unit
Method Reaction Column
Temperature Setting 50 - 140°C (in increments of 1°C)
Photometer
Spectrometer Aplanar concave diffraction grating
Wavelength 570 nm, 440 nm
Miscellaneous
Dimensions & Weight (excluding PC) 900 x 650 x 1050 mm ( W x D x H)
Weight (excluding PC) Approximately 250 kg
Operating Temperature 15-35°C
Power Requirements 115V, 220V ±10%, 1500VA (50/60 Hz)
Chromatography Software Full instrument control and data processing via EZChrom EliteTM for the L-8900 Amino Acid Analyzer
Ordering Information
L8900-PH Protein Hydrolysate Analysis System
L8900-PF Physiological Fluid Analysis System
*
EZChrom Elite is a trademark of Agilent Technologies.

Features

Automatic Pulsation Dampening

Pulsation dampening diagram

A high-speed, real-time feedback control system ensures excellent flow rate stability. This dual processor system allows small pressure changes to be detected and the pressure adjusted to maintain stability.

Superb Separation with 3ŵm Particles

Separation chart

The finer the packing particles, the higher the number of theoretical plates. That means better separation. Finer particules also boost flow rate at the maximum number of theoretical plates. The 3ŵm packing particles used in the L-8900 are a key technology for fast analysis over a broad range of flow rates with excellent separation.

Detachable Sample Rack

Detachable sample rack
The sample rack may be quickly removed for easy exchange of sample tubes.

Options

Columns and Buffers for Physiological Fluid Analysis

Columns

Fluorescence Analysis

Cooling Unit

Auto-Sampler Cooling Unit

Maintains integrity for heat labile samples while in the instrument autosampler.

Library

Application Notes

Note Number:
20130124
The production of Parmigiano-Reggiano is tightly regulated, resulting in a cheese of consistently high quality and cost. A lengthy aging process helps impart the cheese with a distinctive taste and texture, with additional value placed on cheeses aged over 18, 22, and 30 months. The maturation process includes enzymatic conversion of amino acids, and, as such, the levels of these amino acids have been found to be correlated with the age of the cheese. Presented here is a chromatographic method for amino acid analysis of Parmigiano-Reggiano using the Hitachi L-8900 Amino Acid Analyzer.

Note Number:
L56
Physiological fluid analyses require a high separability because they involve a number of analytes. Compared here are the results of analyses of a beer sample with a standard column (4.6 x 60mm) and a high-resolution column (4.6 x 80mm).

Note Number:
20121018
The Hitachi L-8900 Amino Acid Analyzer is a stand-alone instrument capable of analyzing amino acids in a wide variety of matrices. Presented here is a summary of the performance parameters for the L-8900, including sensitivity, reproducibility, and linearity, as well as a representative analysis of the amino acids in hydrolyzed soy flour.

Note Number:
Analysis of biological fluid amino acids with an amino acid analyzer usually targets 41 amino acid components. In recent years, however, the study of metabolic errors in newborns, for example, has advanced significantly giving rise to the demand for analysis of more amino acids that induce metabolic errors. To cope with this, new methodology has been developed for the simultaneous analysis of an additional 12 amino acids involved in metabolic errors besides the usual 41 components, thus analyzing 53 components total.

Note Number:
LC59
Desmosine and isodesmosine are known as unique amino acids present in elastin. In the conventional physiological fluid amino acid analysis, their retention times overlap that of ornithine (Orn.), thus making it impossible to perform quantitative analyses. In order to solve this problem, we have used an OPA pre-column labeling method to analyze desmosine and isodesmosine.

Note Number:
20111027
The analysis of phosphorylated amino acids is shown using the Hitachi Amino Acid Analyzer.

Note Number:
20130419
Glucosamine and galactosamine are amino sugars that are found in acetyl forms in biological materials such as bacterial cell walls, insect and crustacean outer coverings, and human red blood antigens. They are also components of supplements for athletes and arthritis patients. Here we describe a method to analyze glucosamine, galactosamine, and alanine with the L-8900 Hitachi Amino Acid Analyzer.

Note Number:
LC67
An analysis method of 21 standard amno acid constituents has been developed. In this method, separation of MetSON and Asp is better than before and an internal standard substance norleucine (Nle) is added in order to accurately analyze a sample oxidized by performic acid. In addition, two more analysis methods have been developed and introduced in this document. These 3 methods use the same column and eluent, enabling analysis of various samples without column exchange.

Note Number:
Demands for analyses of amino acids that induce metabolic errors continue to exist. Recently, a new methodology was created to simultaneously analyze 50 physiological amino acids utilizing a unique column configuration that affords extremely low back-pressure while maintaining the necessary high resolution, thus alleviating much of the stress that traditional columns must endure. The same system configuration also can be used to analyze amino acids related to specific metabolic errors in a shortened time.

Note Number:
Demands for analysis of amino acids that induce metabolic errors continue to exist. Model L-8900, the latest generation in a long line of Amino Acid Analyzers from Hitachi, is a dedicated system capable of simultaneously identifying and quantifying fifty (50) physiological amino acids for the purpose of clinical diagnosis. Unparalleled performance is achieved via a high-resolution 3 µm ion exchange resin, a patented reaction column to eliminate band broadening, and a unique ninhydrin delivery system with no pre-mixing required.

Literature

The L-8900 Amino Acid Analyzer is a dedicated system that continues the long line of Hitachi amino acid systems.