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Affordable and robust HPLC system engineered for seamless integration and reliable analyses
High Performance Liquid Chromatography (HPLC) is utilized in a diverse range of fields, including medicine, food production, chemistry, and environmental science, making HPLC systems increasingly in-demand among the various types of analytical equipment. The Hitachi HPLC Primaide is designed for long-term, stable operation, and features both high reliability and great durability. It offers tremendous capability for everyday analysis.
Based on over 50 years of experience in the development and production of HPLC systems, Hitachi proudly introduces the Primaide. Make the most of your analytical equipment with Hitachi as the “Prime Aide” in your lab!
GC : (-)-Gallocatechin, EGC : (-)-Epigallocatechin, C : (-)-Catechin, EGCG : (-)-Epigallocatechingallate, EC : (-)-Epicatechin, GCG : (-)-Gallogatechingallate, CG : (-)-Catechingallate
|Peak No.||Component||Retention Time|
|Average / min.||R.S.D. / %|
|Column||HITACHI LaChrom C18 (5 µm) 4.6 mm I.D.×150 mm L.|
|Mobile phase||A : CH3OH B : H2O|
|Gradient program||0 - 11 min A 65 → 100%|
|Flow rate||1.0 mL/min|
|Detection||UV 265 nm|
|Injection vol.||10 µL (100 g/mL each.)|
Injection Volume Reproducibility
|Mobile phase||CH3OH : H2O = 60 : 40|
|Flow rate||1.0 mL/min|
|Detection||UV 265 nm|
Organizer capable of accommodating various solvent bottles
The organizer can accept the simultaneous mounting of the following solvent bottles.
|1||3.785 L (U.S. gallon bottle) × 2 + 500 mL × 2|
|2||1.0 L × 5|
Organizer also doubles as a power supply module
The organizer, which is also a power supply module, supplies power to one pump, one autosampler, one detector (one UV detector, or one Diode array detector),and one interface control board.
Here are some latest applications measured by Primaide.
Analysis methods of organic acids include UV detection (detection of absorption of carboxyl groups), electrical conductivity detection (detection of ionic compounds), a method using pH indicators (visible absorption detection of pH changes caused by acidic components) and a post-column derivatization method for high selectivity of detection. An appropriate method must be selected based on analysis samples and purposes.
The following describes examples of analysis of organic acids using a portable UV detection system and the reversed-phase column, "LaChrom C18-AQ".
Phenoxyethanol is often used in combination with parabens in cosmetics to reduce the added paraben content.
In this presentation, we will introduce and examine the simultaneous analysis of phenoxyethanol and parabens.
Glycosides are mainly O-glycosides, sugar derivatives with various physiological activities that are widely distributed in plants.
Glycosides are also widely known as components of crude drugs. Presented here is the analysis of the glycosides swertiamarin, puerarin, paeoniflorin, sennoside, and glycyrrhizinic acid using HPLC with diode array detector (DAD).
Multiple peaks of various components are detected in the analysis of a crude drug. By using DAD, the UV absorption spectra of the target component and the Standard Sample can be compared and confirmed and therefore, more accurate quantitative analysis is possible.
Please take care to note that since vitamin C and erythorbic acid are unstable, they are easily decomposed during sample preparation or with the passage of time, and that it is difficult to obtain linearity and reproducibility. Therefore, these analysis conditions are suitable for qualitative analysis. For quantitative analysis, the use of an individual test method for each vitamin is recommended.
Examples of analyses for aspartame in (1) carbonated drink and (2) apple cider vinegar drink are introduced here.
Six dyes analyzed here have maximum UV absorption at different wavelengths and therefore, DAD (diode array detector) was used to analyze them simultaneously. By using DAD, a chromatogram can be extracted at the optimal wavelength for each dye.
As a spectrum of a standard sample can be compared with that of a target component for confirmation, more accurate quantitative analysis is possible.
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.
Introducing the measurement example using liquid chromatograph (HPLC) in various different fields.
The basics of HPLC, from "Principles and system configuration" to "Feature of various detection methods."
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