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Hitachi

Hitachi High-Technologies GLOBAL

Ultra High Performance Liquid Chromatograph ChromasterUltra Rs

A new UHPLC, designed for a new era, has emerged to lend indispensable support to your research.

We set out to view the issues from a new angle; visualizing the future with no preconceptions. Toward the realization of high resolution and high sensitivity, achieved through advanced technologies, our daring approach resulted in the ChromasterUltra Rs.
The future of UHPLC starts here and now.

Features

High Resolution

The newly developed LaChromUltra II column is the longest UHPLC column with a 250 mm length. When using together with the reduced volume system ChromasterUltra Rs, exceptional high separation performance can be obtained.

  • * Each column has its own recommended flow rate range for analysis, and it is advisable to respect these limits in order to maintain and optimize the separation performance of any column.

The combination of this column and the ChromasterUltra Rs will enable a system pressure tolerance of 140 MPa with 50,000 or more theoretical plates per column.

High Sensitivity

A total reflection type capillary flow cell is incorporated into the diode array detector to cope with the increasing need for high sensitivity analyses of hazardous substances, genotoxic impurities, etc.
A high sensitivity flow cell with an optical path length of 65 mm is also available. In addition to high sensitivity analyses, carryover is reduced through the use of a double corkscrew mixer.

High sensitivity total reflection type capillary flow cell

The diode array detector (DAD) exhibits low noise and low drift, achieved through the use of a new optical system providing optimal conditions for high sensitivity analysis. The optional high sensitivity flow cell (optical path length of 65 mm) further enhances, sensitivity; about a 10-fold increase is obtained compared with our previous model (LaChromUltra), thus making possible applications including impurities from side-reactions, genotoxic impurities, etc.
The acquisition of impurity profiles during all stages of synthesis and in raw materials used in medicines and chemicals, intermediate by-products, and finished drugs would be one of the useful applications.

Double corkscrew mixer

The Binary pump is equipped with the latest design in microfluidic double corkscrew mixers.
An efficient mixture is attained even for a low volume, resulting in a baseline that is extremely stable during gradient analysis, enabling higher sensitivity analysis.

Structure of double corkscrew mixer:
Repeated branching and merging of channels within the mixer provide effective solvent mixing (mixer volume of 55 µL).

[Conditions]
Column LaChromUltra II C18(1.9 µm) 2.0 mm I.D.×50 mm L.
Eluent A)0.1% TFA/H2O(v/v)
B)0.1% TFA/CH3CN(v/v)
A/B=50/50
Flow rate 0.500 mL/min
Detection UV 214 nm

Carry-over

Low carry-over (0.001% or less) is attained via optimized injection port structure.

  • The dead volume of the injection port is reduced
  • High flow rate washing is achieved through the use of a washing pump dedicated to the autosampler
  • Two solvents are available for washing the needle inner wall as well as the inside of the injection valve
  • A back flush function for the injection port is newly incorporated

Usability

GUI Controller (optional)

All modules can be controlled from the Graphical User Interface (GUI) controller.
The GUI is comprised of a color LCD monitor (5.7-inch color TFT display with LED back-light) and a touch panel made for easy viewing and simple operation.
Up to 10 programs including a timer function, pre-analysis tasks (Wakeup), and post-analysis tasks (Sleep) can be created for a system.
The GUI controller enables you to check the status of consumables usage for all units that are connected to the system.

MEM Column fitting (optional)

Hitachi' s own Moment Enhancing Mechanism (MEM) column fitting is a simple but exciting new development.
The finger-tight piping integral fitting, which has a very small dead volume, is capable of safely tolerating system pressures as high as 140 MPa.


MEM column fitting


A view of the operation of attaching a column

Lineup

  • Diode array detector (DAD) system
  • UV-VIS detector system

Data

Stable solvent delivery allows a board range of analyses from UHPLC to HPLC

Stable solvent delivery is realized, independent of pressure and solvent composition, by incorporating LBT control and by correcting the bulk modulus of the solvent.
Stable analyses are achieved with 5 µm columns, 3 µm columns, and 1.9 µm columns.

Excellent reproducibility

Even for gradient analysis

under high pressure conditions, highly reproducible and high resolution results are achieved.


Highly reproducible analyses are also possible for analyses of protein digests, which produce numerous peptide peaks.

High resolution analyses of related substances (impurities)

Erythromycin (a macrolide antibiotic having a basic structure comprised of a 14-membered ring) is used as a sample model for separation of the main component and related substances to compare the separation using an HPLC column vs. the separation using a UHPLC column.

HPLC column [Conditions]
Column LaChrom II C18(5 µm) 4.6 mm I.D.×150 mm L.
Flow rate 1.0 mL/min
Eluent 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15
Detection DAD 210 nm
UHPLC column [Conditions]
Column LaChromUltra II C18(1.9 µm) 3.0 mm I.D.×250 mm L.
Flow rate 0.710 mL/min
Eluent 20 mmol/L Phosphate Buffer/CH3CN/CH3OH=45/40/15
Detection DAD 210 nm


More satisfactory separations are attained by the use of the newly developed high resolution column for UHPLC applications.

Applications

Introducing the measurement example using liquid chromatograph (HPLC) in various different fields.

HPLC Basic Course

The basics of HPLC, from "Principles and system configuration" to "Feature of various detection methods."

Development of UHPLC

Introducing the interview with three engineers who involved in the development and design of Hitachi UHPLC.

Science Ring

The trademark that represents our strong bond with the customer and shows our pledge to connect science and society to create new value.

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