Skip to main content

Hitachi

Hitachi High-Technologies GLOBAL

Drugs that contain a steroid skeleton include Prednisolone, Betamethasone, Dexamethasone, and Hydrocortisone, and each possesses a different duration of action and strength.
Steroid drugs providing powerful anti-inflammatory and immune suppression actions are used in the treatment of various illnesses, including allergy and inflammation ailments.
However, when administered continuously, steroid drugs can potentially trigger a variety of side-effects, such as obesity, high blood pressure, and diabetes. In view of this fact, when using steroids, treatment methods are needed that minimize side-effects through the proper adjustment of dosage, as well as methods of administration in a manner that is appropriate for a given disease.
The text below describes analyses that were performed on two types of off-the-shelf ointments.

Analysis of steroid drugs

Samples

Commercially available ointments A and B

Sample preparation

Standard samples

System configuration

5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System

Conditions

Column HITACHI LaChrom C18 (5 µm) (4.6 mmI.D. x 150 mm)
Mobile phase H2O/MeOH = 40/60 (V/V)
Flow rate 1.0 mL/min
Column Temperature 40°C
Detection 254 nm
Injection vol. 10 µL

Results of standard sample assaying

Linearity

(Range of calibration: 0.1 - 20 µg/mL)

Conditions

Column HITACHI LaChrom C18 (5 µm) (4.6 mmI.D. x 150 mm)
Mobile phase H2O/MeOH = 40/60 (V/V)
Flow rate 1.0 mL/min
Column Temperature 40°C
Detection 254 nm
Injection vol. 10 µL

Results of analysis

As a result of analyzing two off-the-shelf ointments, hydrocortisone acetate was detected in Ointment A, and Prednisolone in Ointment B.

NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.

Donwload Adobe Reader
In order to read a PDF file, you need to have Adobe® Reader®