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Hitachi High-Technologies GLOBAL

Anion surfactants interfere with wastewater treatment processes due to their foaming properties. A standard analysis mixture consists of five sodium alkylbenzenesulfonates of varying alkyl chain length (C10-C14) at a concentration of 0.004 mg/L each, for a total surfactant concentration of 0.02 mg/L. The method includes a solid phase extraction step followed by HPLC analysis, and is described in Table 24 of the bulletin. The data describes the analysis of a five component mixture of sodium alkylbenzenesulfonates at a concentration of 0.004 mg/L each, concentrated 250-fold to a concentration of 1 mg/L in a pretreatment step.

Analysis of anion surfactants

Sample

Alkyl benzenesulfonate (the active ingredient of a synthetic detergent)

Solid phase extraction procedure

Solid phase extraction procedure

*1:
NOBIAS RP-OD1

This is a reverse-phase polymer-based column, and it is a solid-phase packed column in which an octadecyl silyl group is linked to a hydrophilic methacrylate substrate.

System configuration

5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System

Conditions

Column HITACHI -Inertsil ODS-3 (3 µm) (4.6 mmI.D. x 150 mm)
Elute 0.1 mol/L sodium perchlorate - (CH3CN : H2O = 65 : 35)
Flow rate 0.6 mL/min
Column Temperature 40°C
Detection FL Ex 221 nm, Em 284 nm
Injection vol. 20 µL

Results of measurement of standard samples

C10BS: Sodium Decylbenzenesulfonate
C11BS: Sodium Undecylbenzenesulfonate
C12BS: Sodium Dodecylbenzenesulfonate
C13BS: Sodium Tridecylbenzenesulfonate
C14BS: Sodium Tetradecylbenzenesulfonate

The toluene used in the pretreatment for the analysis of a non-ionic surfactant, another test item, can affect the analysis of the anion surfactant.
An evaluation of elution conditions indicates that toluene is eluted between C12 and C13; therefore, high-precision analyses with any impact on quantitation are possible

Verification of separation from toluene

Conditions

Column HITACHI -Inertsil ODS-3 (3 µm) (4.6 mmI.D. x 150 mm)
Elute 0.1 mol/L sodium perchlorate - (CH3CN : H2O = 65 : 35)
Flow rate 0.6 mL/min
Column Temperature 40°C
Detection FL Ex 221 nm, Em 284 nm
Injection vol. 20 µL

NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.

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