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© Katsuji Ito, Manabu Shirai, Mami Konomi, Eiko Nakazawa (Hitachi High-Technologies Corporation)
© Sumire Inaga (Tottori University, Faculty of Medicine)
Three dimensionally reconstructed images of a cultured HeLa cell stained with platinum blue are presented; the images are composed of cross-section images serially acquired with 10 nm steps using an FIB-SEM.
Morphologies of chromosomes or cytoplasmic processes and their dispersion states in the cell are clearly visualized. It is expected that higher-order structure of unknown chromosomes will be elucidated by this technique.
1st Prize. At 73th photo contest hosted by the Japanese Society of Microscopy in 2017.