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High-Performance Liquid Chromatograph Chromaster® 5610 MS Detector

High-Performance Liquid Chromatograph Chromaster® 5610 MS Detector

Easy Acquisition of Mass Information!

The Hitachi Chromaster 5610 mass detector is designed for HPLC applications, that may not require a large-scale mass spectrometer.

  • Improved chromatogram reliability
  • Easy acquisition of mass information
  • No need for special installation environment
  • Easily maintained
*:
"Chromaster" is a registered trademark of Hitachi High-Tech Corporation in Germany and France.

Features

Compact tabletop design - Ideal footprint for any HPLC laboratory

  • With its space-saving compact design, its footprint is equivalent to that of a HPLC system.
  • The N2 gas usage has been reduced (maximum flow rate of 3.4 L/min).
    The system can be operated with a N2 gas cylinder.
  • Can be connected to an existing HPLC (inquire about the compatibility).

Site Requirements

Usability Equivalent to Optical HPLC Detectors

Instrument and method settings
Ease of use is achieved by eliminating complicated MS settings for those new to mass analysis.
The Chromaster 5610 is equipped with an auto-tuning function to provide optimized instrument conditions to help HPLC users who are unfamiliar with the operation of mass spectrometers.
Measurement Results
Functions such as the contour display allow the verification of an overall chromatographic elution pattern. The user interface resembles that of a PDA detector so operations are as simple as using HPLC detectors.


Measurement result by scanning mode

Maintainability Equivalent to Optical HPLC Detectors

Maintenance
When reduced sensitivity or contamination is suspected, the atmospheric-pressure ion filter can be removed for easy cleaning.
As the vacuum pump can keep running while the filter is being cleaned, the measurements can be started immediately after the maintenance.

Procedure for removal of the atmospheric filter

The atmospheric ion filter can be removed in 3 steps, and it can be reused after ultrasonic cleaning.

Specifications

Specifications
ItemsDescription
Measurement mass range (m/z) 20 - 1,000
Ion source Electrospray ionization (ESI)
Dimensions and Weight 440(W) × 600(D) × 430(H) mm / Approx. 51 kg
N2 gas usage Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa

Data

FIA*¹ Measurement (with autosampler and pump)

As the mobile phase is delivered by a pump, a small volume of sample is injected and measured by “Flow Injection Analysis Method”. By connecting the HPLC to an autosampler, the measurement can be automated and it can be used for the confirmation of mass information in each sample.

*1
Flow injection analysis

Monitoring of Mass Information for Compounds

Confirmation of the mass information for a compound is easier,
By simply setting the sample(s) to be analyzed in the autosampler, the mass information can be obtained. Monitoring a sequence analysis is also available.

  • * This sample was provided by the Department of Pharmaceutical Molecular Design, Meiji Pharmaceutical University.
  • * FIA sequence analysis such as the monitoring introduced here, the UI pad or GUI controller is required to control the HPLC module.

LC-MS Measurement

During a HPLC experiment, by adding the mass spectral information collected by the MS detector to the measurement data obtained by the UV (or similar) detector, the reliability of the identification result is enhanced. In addition, the SIM (selected ion monitoring) measurement improves the selectivity for the target compound. The method is particularly useful for the measurement of non-UV-absorbing samples.

Measurement of bacterial culture

By connecting the diode array detector and MS detector in series, the UV spectrum and mass spectrum can be simultaneously obtained.
With this system configuration, a large amount of sample information can be obtained. In addition, the measurement result obtained by the scanning mode of the MS detector is shown in the form of a contour display, which is commonly used for displaying data from diode array detectors. Therefore, the identity of the detected ion can be more accurately determined.


Measurement Result by Diode Array Detector


Measurement Result by 5610 MS Detector

  • * The data introduced here was provided by the Department of Microbial Chemistry, Kitasato University.
  • * These data are an example of measurement; the individual values cannot be guaranteed.

Applications

Introducing the measurement example using liquid chromatograph (HPLC) in various different fields.

HPLC Basic Course

The basics of HPLC, from "Principles and system configuration" to "Feature of various detection methods."

Science Ring

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