Compact CE Sequencer DS3000
A compact, convenient, easy-to-use DNA sequencer
The DS3000 inherits the same core capillary electrophoresis technology developed by Hitachi High-Tech over many decades – now in a compact, easier than ever to use, system.
Sequencing analysis and fragment analysis can be performed easily.
Sales area: Japan, China, Korea, Taiwan(*)
*Sales starts when ready
Features
Intuitive and easy-to-use touch screen panel
The screen is equipped with a touch-panel GUI that saves space and improves operability. System management and run conditions can be reviewed at a glance simplifying the genetic analyses process.
The intuitively designed touch screen panel puts ease of use and operability first.
The four main operations of Run Measurement, Review Results, Maintenance, and Protocols are clearly displayed.
The detail of each operation is available at a single touch.
Easy to install cartridge system
The DS3000 adopts easily installable cartridges for the capillaries and reagents.
Reduced maintenance frequency
-
No need to clean a pump
By developing a syringe-type polymer cartridge having a high-durability movable seal, and making the liquid transport system pump-less, eliminates pump maintenance. -
No need to remove bubbles
By adopting an unique polymer injection system, it is not necessary to remove bubbles at the time of polymer filling, reducing excess polymer consumption. -
Less frequent light source replacement
Pulse signal control means LD is only on during detection. The shortened illumination time increases the useful lifespan, especially when compared to complicated and expensive light sources such as Argon gas lasers.
Reduced footprint
The incorporated touch screen has reduced the DS3000 to the compact dimensions of 400 mm wide × 600 mm deep × 600 mm high.
Enhanced user convenience
-
Equipped with remote monitoring system
By incorporating a remote monitoring system, it is possible to monitor the instrument state, set assay conditions, display analysis results, and prepare reports from a Web browser. -
Utilizes an ordinary general-purpose PC to reducing set-up costs
The instrument can simply be added to existing networks with secondary analysis and reporting allocated to a standard PC.
Application [Sequencing analysis]
Contiguous Read Length
Sequencing was performed using the BigDye® Terminator v3.1 Sequencing Standard Kit in Standard Sequencing mode. The electropherogram data display demonstrates high resolution and accurate QV values were maintained at 500 bp. Contiguous Read Length of 600 bp or higher in Fast Sequencing mode, or at 700 bp or higher in Standard Sequencing are typical and reproducible.
Application [Fragment analysis]
Human Identification (HID)
200 pg of 2800 M control DNA was amplified with a PowerPlexTM Fusion 6C System, and subjected with the Promega® 6 dye system compliant electrophoresis module. The obtained (fsa), file was analyzed and visualized by a secnodary software, GeneMarker HID.
* The electropherogram view is obtained from GeneMarker HID (SoftGenetics, LLC, sold separately)
Specifications
| Item | Details |
|---|---|
| Number of capillaries | 4 |
| Capillary length | 36 cm |
| Sample format | 8-tube strip × 4 |
| Device control | Touch panel PC |
| Number of Dyes | 6 |
| Application | Sequencing analysis / Fragment analysis |
| Size | 400 (W) × 600 (D) × 600 (H) mm |
| Weight | 45 kg |
| Performance guarantee temperature | 15 – 30°C |
| Performance guarantee humidity | 20 – 80% RH (no condensation) |
| Power input | 100 – 240 ±10％ VAC,50/60 Hz |
| Rated power | 260 VA |
| Supported secondary analysis software |
|
| Run Module | Application | Polymer type |
Contiguous Read Length*1 (bp, QV20 CRL) |
Average run time (minutes) |
|---|---|---|---|---|
| Fast_Sequence36_Polymer7 | Sequencing analysis | Polymer7 | ≥600 | ≤32 |
| Standard_Sequence36_Polymer7 | Sequencing analysis | Polymer7 | ≥700 | ≤60 |
| BDx_Fast_Sequence36_Polymer7 | BDx sequencing analysis | Polymer7 | ≥600 | ≤32 |
| BDx_Standard_Sequence36_Polymer7 | BDx sequencing analysis | Polymer7 | ≥700 | ≤60 |
| Run Module | Application | Polymer type |
Average run time (minutes) |
Sizing precision*2 (bp,50～400bp) |
|---|---|---|---|---|
| Fragment_Analysis36_Polymer7 | Fragment analysis | Polymer7 | ≤35 | NA |
| Fragment_Analysis36_Polymer4 | Fragment analysis | Polymer4 | ≤44 | <0.16 |
| Product name | Part number | Details | Remarks |
|---|---|---|---|
| Capillary Cartridge 36 cm | 613-0330 | 1 pcs | Storage temperature: 15 – 30°C |
| Buffer | 613-0252 | Anode Buffer × 2 cartridges Cathode Buffer × 2 cartridges |
Storage temperature: 2 – 10°C |
| Polymer7 | 613-0251 | 4 cartridges | Storage temperature: 2 – 10°C |
| Polymer4 | 613-0250 | 4 cartridges | Storage temperature: 2 – 10°C |
| Septa for Cathode Buffer Cartridge | 613-7231 | 10 pcs | |
| Retainer for Cathode Buffer Cartridge | 613-7233 | 4 pcs | |
| Septa for 8 well tubes | 613-7230 | 24 pcs | |
| Base and Retainer for 8 well tubes | 613-7232 | 4 pcs | |
| Anode Electrode Assembly | 613-7263 | 1 pcs |
*1 Contiguous Read Length (bp, QV20 CRL) is measured with BigDye® Terminator v3.1. Sequencing Standard Kit (Thermo Fisher Scientific, sold separately)
*2 Sizing precision (bp,50～400bp) is measured with PowerPlexTM ESI17 Fast Allelic Ladder and WEN ILS 500 ESS. (Promega, sold separately)
* BigDye is a registered trademark of Thermo Fisher Scientific Inc.
* Promega, PowerPlex is a registered trademark of Promega Corporation.
