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The standard installation of UH5300 spectrophotometer includes an automatic 6-cell turret. When analyzing many samples, the increased work efficiency can be expected by using the "intelligent Start" function. UH5300 spectrophotometer also focuses on the absorbance differences between cells. All cells are measured for the auto-zero values, which are recorded as the correction values. Therefore, the instrument is capable of handling the analysis where a slight absorbance difference is being measured. This time, Cr(VI) in water samples was analyzed by using this function. As a result of the analysis, a calibration curve with a high correlation coefficient (R2= 0.9999) was obtained.