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  4. Application Notes: Analytical Systems
  5. Application Notes: Fluorescence Spectrophotometers (FL)
  6. Fluorescence Fingerprint (EEM)
  7. Fluorescence Fingerprint (EEM) Measurement Procedure

Fluorescence Fingerprint (EEM) Measurement Procedure

(1) Sampling

Fluorescence fingerprints can be obtained from not only liquid samples but solid samples as well.

Liquid samples

  • Liquid (low viscosity): Raw water, etc.
  • Liquid (high viscosity): Oil, eggs, milk, etc.
  • Paste: Mayonnaise, cream, etc.

Solid samples

  • Powder: Grain powder, spices, etc.
  • Granular: Rice, beans, supplements, etc.
  • Plate-like (cut): Meat, fruit, glass, plastic, etc.

(2) Preparation

A bare minimum number of preparation steps such as filtration, centrifugation, dilution, pulverization and homogenization are necessary.

Liquid samples

  • Filtration
  • Centrifugation
  • Dilution
    → Results in a uniform sample condition

Solid samples

  • Pulverization, homogenization:
    pulverizer → sieve
  • Pelletization:
    pulverizer → tableting machine
  • Cutting
    → Produces a uniform sample surface

(3) Sample mounting

Depending on the samples characteristics, different arrangements are used:

Liquid samples

  • Liquid (low viscosity): 10 mm square cell
  • Liquid (high viscosity): wide cell + solid sample holder
  • Paste: wide cell + solid sample holder

Solid samples

  • Powder: powder cell + solid sample holder
  • Granular: wide cell + integrating sphere
  • Plate-like (cut): quartz plate + solid sample holder

(4) Measuring operation

(5) Data processing

Set the measurement conditions, record the sample names and make measurements in accordance with the table. Analyze measurement data with multivariate analysis software.


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