F-7000 Fluorescence Spectrophotometer applications in various field
Industrial Material Field
Measurement of fluorescent materials
Measurement of fluorescence quantum yield of solid sample
The Hitachi F-7000 Fluorescence Spectrophotometer with accessories allows for calculation of the fluorescence quantum yield of a solid sample from measurements.
The high-level basic functions of the F-7000, such as high sensitivity at S/N 800 or more and a dynamic range up to the sixth or higher orders, brings more power.
<Required system configuration>
- F-7000 Fluorescence Spectrophotometer
- Integrating sphere
- Spectrum correction kit
* Integrating sphere is optional.
* For correction over 600 nm, the R928F Photomultiplier and a substandard light source are required.
<Measurement of fluorescence quantum yield of solid samples>
The quantum yield of tris 8-quinolinolato aluminum complex (Alq3) powder, an organic electroluminescence material, can be obtained from the spectrum (right figure) of a standard reflective material (aluminum oxide auxiliary white sheet).
* Quantum yield cannot be calculated on the same software.
Organic EL materia
In this example, the F-7000 was used to analyze the luminescent characteristic of trisaluminum complex powder used as a luminescent material for organic EL display.
A solid sample holder, its powder cell, the photomultiplier R928F, and the filter set were use.
Scan speed: 12,000 nm/min
Excitation slit: 5.0 nm
Emission slit: 5.0 nm
Photomultiplier voltage: 400 V
Spectrum correction: Activated
Beam-cut filter (UV-39) used Photomultiplier R928F used
The acquisition of these data was made possible by the three-dimensional measurement function and high-speed scanning capability of the F-7000.
Rare earth element complex (Eu chelate)
The example below shows the phosphorescence spectrum and lifetime measurement of the Eu(tta)3(TOPO)2 complex, a rare earth element.
With the F-7000, the analysis of phosphorescence life of 1 ms order can be performed at room temperature without special accessories.
Measurement of intermolecular actions
FRET (Fluorescence Resonance Energy Transfer) and BRET (Bioluminescence Resonance Energy Transfer)
The Model F-7000 can measure the intermolecular activities such as FRET and BRET.
Measurement of calcium in cell
Ca2+ concentration in cells
With the optional interacellular calcium measurement accessory, the F-7000 can measure fluorescence intensity values at two wavelengths in EGF-injected COS-7 cells (extracted from a monkey's kidney), and calculate the concentrations of Ca2+.
The sample was a cultivated cell fluorescence-labeled by Fura2-AM.
The change in Ca2+concentrations in the live cell was also measured. During this analysis, the EGF receptor appeared in the COS-7 as the Ca2+ level increased due to EGF injection.
The Model F-7000 can measure biological samples with higher sensitivity and speed.
Environmental pollutants can be identified by comparison of three-dimensional fluorescence spectra.
The conventional three-dimensional measurement function has been made more sophisticated by F-7000, enabling high-speed and high-accuracy measurement.