Fluorescence Spectrophotometer F-2710
High sensitivity achieved by adopting a high-brightness Xe lamp and optimizing the signal processing system.
Features
1. Achieved Higher Sensitivity
The F-2710 achieves higher sensitivity with an S/N (P-P) of over 300 and an S/N (RMS) of over 1,000 by adopting a high-brightness xenon lamp and optimizing the signal processing system.
Example of S/N Measurement Results
Calibration relationship of very low concentration fluorescein solution
HITACHI's unique high-efficiency diffraction grating and low-noise detection system allow for the measurement of very low concentrations, resulting in a system that effectively measures small amounts of ingredients or samples. The acquired fluorescence intensity is displayed in the range from 0.001 to 9999. Even with low fluorescence intensity a superior calibration relationship can be achieved, and very small amounts of fluorescein, on the order of 1 x 10-12 mol/L, can be detected. As shown here, the F-2710 produces a superior calibration relationship with a coefficient of determination of 0.9998 in the very low concentration range from 1 x 10-12 to 1 x 10-10 mol/L.
2. Standard Long-Life Light Source
The light source is equipped with a long-life type xenon lamp as standard, achieving a light source lifespan (usage limit time) of 2,500 hours, which is five times longer than the standard equipped lamp of the previous model F-2700 (500 hours).
3. Abundant Accessories
More than 30 types of abundant accessories, which were well-received with the F-2700, are available. By utilizing accessories tailored to the measurement sample and analysis purpose, it is possible to respond to measurements in various fields.
Specifications
| Item | Description |
|---|---|
| Light source | 150W Xenon lamp (self-deozonating lamp house) |
| Photometric principle | Monochromatic light monitoring ration calculation |
| Sensitivity | 1,000 or more (RMS) (bandpass 5nm, response time 2 S) |
| Bandpass | 2.5, 5, 10, 20 nm (both Ex and Em) |
| Wavelength scan speed | 60～12,000 nm/min (five steps) |
| Dimensions / Weight | 600 (w) x 503 (D) x 343 (H) mm/ approx.41 kg |
| Power consumption | AC100, 115, 220, 230, 240 V 50/60 Hz 400 VA |
Fluorescein Measurement
●Measurement of humic substances
3D fluorescence spectra of humic substances in mineral water
Humic substances are measured as an index of the organic compounds existing in water.
With a very high sensitivity of the F-2700, it is possible to measure organic compounds existing in mineral water at very low concentrations.
●Measurement of chlorophyll a
Calibration curve and fluorescence spectra of chlorophyll a
Chlorophyll a is used for the quantification of aquatic phytoplankton, and is measured as a bioactivity index.
Analogous compounds, such as pheophytin a can also be individually measured with high sensitivity by the fluorescence method, due to their difference in excitation and emission spectra.
Citations
Powered by BiozIntroducing the measurement example using spectrofluorophotometer (FL).
The basics of fluorescence spectrophotometers (FL).
