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  6. Fluorescence Spectrophotometers (FL) Basic Course

Fluorescence Spectrophotometers (FL) Basic Course

1. What is fluorescence?

1.1 When are you excited? (Excitation spectrum)
1.2 What happens after being excited? (Fluorescence spectrum)
1.3 What if there are many human clones? (Quantitative measurement)
1.4 What's the difference from absorptiometry?
　1.4.1 Wavelength to measure
　1.4.2 High sensitivity
　1.4.3 Large amount of information
　1.4.4 Shoot at one component
　1.4.5 Weak point of the fluoroscopic method

2. Mechanism of the Spectrofluorophotometer

2.1 General mechanism
2.2 Components to be used
2.3 Precautions for comparison

3. Advanced Measurements

3.1 Spectrum correction
3.2 Three-dimensional measurement
3.3 Measurement of intracellular calcium ion concentration
3.4 Phosphorescence measurement

Appendix

Measurement example for a fluorescence spectrum
Glossary

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