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Hitachi High-Technologies GLOBAL

We introduce you to plenty of data on analytical methods and their results using our Fluorescence Spectrophotometer.

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NO. Title
FL140001 The Classification of the Pineapple product by the Multivariate Analysis using 3-D Fluorescence Spectra Measurement
Fluorescence Spectrophotometer F-7000
FL130004 The Classification of the Starch by 3-D Fluorescence Spectra Measurement (Fluorescence Fingerprint) using the Multivariate Analysis
Fluorescence Spectrophotometer F-7000
FL130002 3D Fluorescence Spectrum of High concentration Samples (Olive oil)
Fluorescence Spectrophotometer F-7000
FL33 ETHANOL MEASUREMENT AND ENZYME ASSAY WITH USE OF AUTOMATIC 4-TURRET ACCESSORY
The F-7000 fluorescence spectrophotometer achieves a high sensitivity (S/N 800:RMS), ultra-high-speed (60,000nm/min) wavelength scan at the highest level in its class, with a wide dynamic range up to 6 digits. Furthermore, it is applied to research, development, and quality control in a broad range of fields, such as industrial materials, life sciences, and biotechnology.
The 4-turret accessory used in this experiment automatically measures, by computer control, the time course of the fluorescence intensity at regular time intervals through 4 rotating cells installed in a turret. A reaction trace for an enzyme is used to measure the time course of fluorescence intensity in plural samples. Previously, the time course was measured for one sample at a time, so the measurement time required was proportional to the number of samples. Application of the automatic 4-turret accessory reduces measurement time.
An enzyme assay was applied to ethanol measurement in this experiment. The amount of ethanol in the fish is increased according to a decline of the freshness, so this measurement is referred to as the freshness index. Ethanol is usually measured with a spectrophotometer. When ethanol is present in trace amounts,
a low concentration measurement is performed with a fluorescence spectrophotometer. In this experiment, the general enzyme assay reagent "F-kit ethanol (J.K.International)" was used. In addition to the enzyme assay, a spectrophotometer was used to measure ethanol amounts. The analysis conditions were optimized, and an analytical curve for a standard solution was created.
FL32 FLUORESCENCE SPECTRUM MEASUREMENT METHOD ALLOWING A COMPARISON OF DIFFERENT EQUIPMENT - SPECTRAL CALIBRATION OF INSTRUMENTAL RESPONSE AND VALIDATION METHOD, AND MEASUREMENT EXAMPLE FOR A STANDARD FLUORESCENT MATERIAL -
Keywords: Fluorescence spectrum, Spectrum calibration, Instrumental response, Rhodamine B, Quinine sulfate, Standard material, GLP/GMP program
FL31 DETECTION OF INTERACTION BETWEEN RECEPTOR PROTEINS DEPENDING ON BIOLUMINESCENCE RESONANCE ENERGY TRANSFER
Model: F-4500
Keywords: Fluorescence, Luminescence, HEK Cell, Protein Interaction, Receptor, Bioluminescence Resonance Energy Transfer, BRET, FRET, Luminescence resonance energy transition, GFP, YFP, ATP, G-protein
FL30 FLUORESCENCE POLARIZATION METHOD APPLIED TO BIOCHEMICAL STUDY - DETERMINATION OF PCR PRODUCT -
Model: F-4500
Keywords: Fluorescence polarization accessory, Biochemistry, DNA, RNA, PCR
FL29 FLUORESCENCE POLARIZATION METHOD APPLIED TO BIOCHEMICAL STUDY
Model: F-4500
Keywords: Fluorescence polarization method, Biochemistry, Molecular weight, Enzyme activity, Trypsin, Cytoplasmic steaming
FL28 AUTOMATIC FLUORESCENCE SPECTROPHOTOMETRIC ASSAYS FROM MICROTITER PLATES
Model: F-4500
Keywords: Trace sample, Mictotiter plates, Enzyme activity measurement, Automation
FL27 HIGHLY SENSITIVE DETERMINATION OF TERBIUM IN YTTRIUM OXIDE
Model: F-4500
Keywords: Fluorescense determination, Terbium, Three-wavelength calculation
FL26 MEASUREMENT EXAMPLES OF FLUORESCENT WHITE SAMPLES
Model: F-4500
Keywords: Fluorescent whitening agent, Whiteness, Spectral reflected radiance factor, Spectral total radiance factor
FL25 AN ADVANCED FLUORESCENCE SPECTROPHOTOMETRIC MEASURING SYSTEM FOR RAPID ACQUISITION OF THREE DIMENSIONAL SPECTRA
Model: F-4500
Keywords: 3-dimensional fluorescence spectra, High speed data acquisition, Petroleum, Serotonin
FL24 THE DETERMINATION OF CORRECTED EXCITATION SPECTRA, CORRECTED EMISSION SPECTRA AND QUANTUM YIELDS
Model: F-4010
Keywords: Spectrum correction, Correction factor, Quantum yield, Quinine sulfate, Emission spectra, Excitation spectra
FL23 MEASUREMENT OF COUMARIN BY FLUORESCENCE ANALYSIS
Model: F-2000
Keywords: Coumarin, Kerosene, Light oil
FL22 MEASUREMENT OF CYTOSOLIC Ca2+ IN THE INITIAL GROWTH OF MOUSE FIBROBLAST CELLS
Model: F-2000
Keywords: PIP2, PDE, IP3, BALB/C 3T3