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Science & Medical SystemsHitachi High-Technologies

Transmission Electron Microscope HT7700

Transmission Electron Microscope HT7700

The HT7700 with Dual-Mode objective lens features superior high-contrast and high-resolution performance together with analytical capabilities for biological and materials science. Digital design and sophisticated automation increase throughput for both novice and advanced users. Maximize contrast by lowering the high voltage through the 120-40 kV range and continue operation seamlessly. Configure the HT7700 to meet all of your advanced sample analysis needs, with customization options for BF-/DF-STEM, EDX, in-situ, cryo-microscopy, and electron tomography.


    The HT7700 features a Dual Mode objective lens that provides both high contrast and high resolution as well as low-magnification modes for optimized imaging of life science and materials science applications.

    User-Friendly Design

    Easy-to-navigate, intuitive user interface. Standard features include automated film stabilization, multi-frame montage, auto-focus, whole grid view, micro-trace, auto-search, low dose, one-touch startup/shutdown, and more.

    Premium Image Quality

    Integrated high-speed camera for seamless navigation. Many high-resolution camera options with superior image acquisition and optimization for specific applications available.

    Outstanding Performance

    A 0.20-nm resolution at 100 kV for the standard pole piece and a 0.14-nm resolution at 120 kV for the EXALENS pole piece. Also provides STEM resolution of 1.5 nm and tilting angles up to ±70°.

    Advanced Applications

    Hitachi 3D Tomography, BF/DF STEM capability, EDX microanalysis, and in situ. Multiple specimen holder options designed for high throughput, heating, cooling, x-ray analysis, rotation, double-tilt, liquid cell, and air protection analyses.

    Environmentally Friendly

    Small footprint, efficient high-voltage tank, and clean turbo-pump vacuum system reduce CO2 emissions by 30% compared to previous models.


    Essential specifications (TEM)

    Essential specifications (TEM)
    Resolution (lattice) 0.204 nm (100 kV)
    [0.144 nm (120 kV) for EXALENS: Optional]
    Magnification Zoom ×200 - ×200,000 (HC mode)
    ×4,000 - ×600,000 (HR mode)
    (Non-rotating zoom system)
    [×200 - ×300,000 (HC mode)×2,000 - ×800,000 (HR mode) for EXALENS: Optional]
    Low Mag ×50 - ×1,000
    Accelerating voltage 40 - 120 kV (100 V/step variable)
    Field rotation ×1,000 - ×40,000 (HC Mode)
    ±90° 15° step
    Specimen stage   Eucentric goniometer stage
    Stage lraverse X, Y: ±1 mm, Z: ±0.3 mm
    Maximum tilt ±30°: Standard, ±70°: Optional
    [±30°, for EXALENS: Optional]
    Main camera 8 M pixel (vertical to horizontal ratio = 3:4)
    Other cameras are also available.
    Standard features Auto focus. Microtrace, Autodrive, Autophoto, features Auto-gun alignment, Live FFT display, Measurement function (manual/automatic distance measurement), Low dose, API (auto pre-irradiation), Scope unit with mild baking function

    Essential specifications (STEM): Optional

    Essential specifications (STEM): Optional
    Image resolulion 1.5 nm (BF-STEM, 100 kV, defined by measuring the gap of sputtered gold particles)
    Detector BF-STEM
    Magnification ×1,000 - ×800,000 (High Mag mode)
    ×1,000 - ×100,000 (Normal mode)
    ×100 - ×2,000 (Low Mag mode)
    Scanning mode Normal scan
    Selected area scan
    Line scan
    Point scan
    Area scan
    Digital scan*1
    Data format TIFF, BMP, JPEG
    Data bit 16 bits (TIFF)
    Image recording resolution 640 × 480 pixel
    1,280 × 960 pixel
    2,560 × 1,920 pixel
    5,120 × 3,840 pixel
    In addition to STEM option, other preparation is necessary.


    Transition to a Complete Digital Solution

    • The functions of the conventional fluorescent screen and binoculars are fully digitized in the TEM control software.
    • Sensitivity has been increased by ~20× compared to the human eye.


    Switch from the search mode (viewing the screen camera) to capturing a high-quality, full-size image by pressing a single button.

    A high-quality image captured from the search area indicated above. A broad selection of large-format cameras is available and can be mounted in several different locations on the microscope to optimize field-of-view vs. magnification.

    Auto Drive Function for High-Speed Recalling of Specific Area of Interest

    All saved images are stored in an image database where the thumbnails of the images can be viewed.

    Each thumbnail image contains the microscope settings used when the image was captured.

    When the operator clicks on any thumbnail image and selects Auto Drive, the specimen stage is automatically driven to the position where the image was captured.

    Image Navigation Using Whole-View and Micro-Trace Function

    The whole-view function allows a whole image of the grid to be acquired and displayed in the stage operation window. The micro-trace function stores and displays the movement path. This allows the operator to easily differentiate between examined and unexamined areas of the specimen.

    Image navigation allows the storage of up to 100 stage coordinate positions and tilt angles. When a saved location is recalled, the instrument drives the specimen stage to the saved coordinate position and tilt angle.

    Auto Pre-Irradiation, API

    API is essential for imaging beam-sensitive specimens and for stabilizing specimens to prevent drift.
    Operators can select from several modes, as shown below (Whole scan, Frame scan, and Area scan), to optimize sample stabilization.
    By using API, the contrast of examined areas (left) matches the surrounding embedded tissue and resin (right). API effectively minimizes damage and specimen drift during observation.

    By means of API, the contrast of examined areas (left) matches the surrounding embedded tissue and resin (right). API effectively minimizes damage and specimen drift during observation.

    Premium Image Quality

    Auto multiple frame function and Auto panoramic function capture and delineate seamless panoramic images for large format results.

    • Continuous images can be collected, saved, and stitched automatically.
    • Images are automatically collected with high precision through the use of image shift or stage shift.
    • The newly developed stitching algorithm combines automatically captured images into one panoramic view with the highest-possible accuracy.

    Outstanding Performance

    Double-Gap Objective lens design for HC and HR

    • Hitachi's unique Double-Gap Objective lens design can be used to generate a long focal length for High Contrast (HC) imaging or a short focal length for High Resolution (HR) imaging.
    • With a simple operation on the software, the HT7700 operator can easily switch between High Contrast (HC) or High Resolution (HR) imaging mode depending on sample requirements.

    Advanced Applications

    Electron Beam Tomography (optional)

    The highly stable Hyper stage of the HT7700 with ±70° automated tilt capability, coupled with Hitachi's unique reconstruction algorithms such as Topography Based Reconstruction (TBR), can be used to create high-quality tomographic reconstructions.

    Double-Axis Tomography (optional)

    The combination of double-axis tomography and Hitachi's unique reconstruction software affords 3D reconstructions with fewer artifacts and more accurate results.

    Scanning Transmission Electron Microscope (STEM) (optional)

    Both Bright-Field (BF) and Dark-Field (DF) detectors can be added to the HT7700 for STEM analysis.
    BF-STEM provides better contrast and image sharpness due to reduced chromatic aberration.
    Furthermore, scattered electrons are detected with high sensitivity by means of DF-STEM, resulting in better contrast for low-Z specimens.
    When an EDX detector is included, multiple elemental maps can be recorded simultaneously.

    High-Resolution Lens EXALENS (optional)

    EXALENS is a newly designed objective lens for the HT7700.
    By achieving a smaller spherical aberration coefficient (Cs), a resolution of 0.144 nm (lattice, accelerating voltage: 120 kV) is obtained.
    EXALENS excels at high-resolution imaging at lower accelerating voltage, facilitating analyses of soft materials, carbon-based nanomaterials, and polymers.

    Specimen Holders for a Variety of Advanced Applications (optional)

    In addition to various Hitachi holders (below), specialty holders for low temperature, heating, in-situ, and other experiments are available from several manufacturers.

    • One Touch Single Tilt Holder
    • Double Tilt Holder
    • One Touch Three Specimen Holder
    • Powder Heating Holder
    • Rotation Holder
    • Three Specimen Holder
    • X-ray Analysis Holder

    Application Data

    Life Science

    Biological sections

    Specimen: Drosophila testes flagella cross sections
    Accelerating voltage: 80 kV
    Courtesy of North Carolina State University

    Specimen: Human Kidney (lupus)
    Accelerating voltage: 80 kV

    Specimen: Human cytomegalovirus
    Accelerating voltage: 80 kV

    Multiple frame montage

    Specimen: Rat sciatic nerve (4M pix camera, 4×4, 16 images);
    Recorded at 100 kV FOV: 16 µm by 16 µm.

    Auto multiple frame software includes a choice of stage or image shift, percent of image overlap and final image size. The montage is automatically adjusted for uniform brightness and contrast.

    Unstained section image

    Unstained section image
    Specimen: Rat sciatic nerve (unstained)
    Accelerating voltage: 80 kV
    Lens mode: HC mode

    Cryo-EM image

    Cryo-EM image
    Specimen: Ice-embedded liposomes
    Accelerating voltage: 120 kV,
    Lens mode: HC mode

    Materials Science

    HR mode image

    HR mode image
    Specimen: Asbestos (anthophyllite)
    Accelerating voltage: 120 kV
    Lens mode: HR mode

    Low Accelerating Voltage Image of EXALENS

    Low Accelerating Voltage Image of EXALENS
    Specimen: Hydroxyapatite
    Acceerating voltage: 40 kV
    Lens mode: HR mode
    Lens: EXALENS

    3D Reconstruction Image from Electron Beam Tomography

    3D Reconstruction Image from Electron Beam Tomography
    Specimen: Carbon-black particles in the rubber
    Accelerating voltage: 120 kV
    Specimen tilt: ±60°
    Tilting step: 2°
    Reconstruction algorithm: TBR



    High resolution image of EXALENS

    High resolution image of EXALENS
    Specimen: Si
    Accelerating voltage: 120 kV

    High resolution image of EXALENS

    High resolution image of EXALENS
    Specimen: 3X NAND Flash Memory
    Accelerating voltage: 120 kV


    Specimen: High Impact Polystyrene (HIPS)
    Accelerating voltage: 100 kV

    Stainless Steel
    Specimen: Stainless Steel
    Thickness: 0.2 µm
    Accelerating voltage: 100 kV


    Hitachi TEM Application Data

    Technical magazine
    "SI NEWS"

    This journal addresses a wide range variety of research papers and useful application data using Hitachi science instruments.


    Photo collections of beauty of metals, minerals, organisms etc. reproduced by the electron microscope and finished more beautifully by computer graphic technology.

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