High-Performance Liquid Chromatograph Primaide PLUS

Features

• Simple operation for any HPLC user
  Even in small to mid-scale facilities, individual operators see increasing opportunities to use multiple HPLC systems. Thanks to its simple design and front-access layout, maintenance of the Primaide PLUS is easy. Even a beginner at LC analysis can confidently operate the Primaide PLUS.
• Excellent basic performance
  The demand for HPLC systems with higher precision, better reproducibility, and improved stability is increasing. The Primaide PLUS is built according to strict specifications to meet such stringent requirements. In addition, labs can apply the Primaide to an even wider range of applications by employing optional units, such as a sophisticated DAD (Diode Array Detector) and an autosampler cooling unit.
• Reliable durability
  Hitachi HPLC systems have earned a reputation as highly durable machines, and the Primaide PLUS is no exception. Our strict production quality standards ensure both high performance and endurance. These standards are applied to all equipment from systems to accessories.

• Optional units mounted inside provide clean appearance
  All principal options, including a degasser, gradient unit, and sample cooling system can be mounted inside the main body. It is not necessary to make room for optional units.
• Front access layout for improved operation and maintenance
  Every module is accessed and handled from the front. Since connections are arranged on the front, it is easy to replace columns and manipulate the flow system. For easier maintenance, the lamp and cell unit are also easily accessible.
• Leak sensors in all modules
  Any leakage in the flow system is detected by leak sensors built into each module. After detection the system halts, ensuring improved safety and reliability.
• Complete control with software
  A control panel is not necessary, as everything can be controlled via the software. By installing a UI- pad (optional), each module can be controlled separately.
• IFC Interface Communication
  More advanced than conventional links, the IFC interface networks all modules, centralizes their communications, digitizes every signal, and enhances both reliability and data-transfer speed.
• GLP function
  A Hg lamp is installed in the detector, and wavelengths in the UV range can be calibrated using its emission line (254 nm) (installed in the Primaide 1410 UV / 1435 DAD / 1440 FL). Each module stores various types of quality assurance information, for improved data reliability.

[Standard samples]
GC : (-)-Gallocatechin, EGC : (-)-Epigallocatechin, C : (-)-Catechin, EGCG : (-)-Epigallocatechingallate, EC : (-)-Epicatechin, GCG : (-)-Gallogatechingallate, CG : (-)-Catechingallate

Module lineup

The Primaide 1110 Pump has been developed for reliable analysis and shares characteristics with Hitachi's existing HPLC pump models. Its consistently high level of performance provides users with strong support in everyday analysis.

• Improvement of flow rate stability
  For pulse compensation, a proprietary real-time feedback method has been developed for Hitachi’s pumps. Not only has the pulse been significantly reduced, but the Primaide 1110 Pump estimates pressure fluctuations and employs the optimal timing to set the plunger speed interval.
• Improvement of retention time reproducibility
  For gradient analysis, injection by the Primaide 1210 Autosampler is synchronized with the movements of the pump. This ensures a highly reproducible retention time, and precise analysis.

The Primaide 1210 Autosampler incorporates direct injection, allowing the automation of consecutive analyses without wasting precious sample.

• Direct injection of small-quantity samples and quicker analysis
  In the direct injection method, the sampling needle becomes part of the flow channel so that the sample in the needle flows into the channel with zero waste. This is a great advantage in the analysis of valuable small-quantity samples. In addition, since the injection method is simpler than using a sampling loop, the injection cycle is shortened by half. This method accelerates throughput and improves efficiency in the analysis of many samples.
• Improved reproducibility results in high-precision analysis
  The syringe for sample measurement is driven by a high-performance motor. The drive improves the reproducibility of injection. Furthermore, peak retention times are also more repeatable due to synchronization of injection with the pump motion, achieving higher analytical reliability.

Injection Volume Reproducibility

• Dark Window Design for accurate and reliable data
  Featuring a newly designed dark window with low light transmittance, the system is compatible with light-sensitive sample analysis, ensuring excellent data reproducibility.

The Primaide 1315 Column Oven is a high-performance column oven that enhances column performance, resulting in sharper peak shape.

• Excellent Temperature Stability
  The column oven adopts the preheat system in which the mobile phase is temperature-controlled immediately before entering the column. The liquid temperature in the column is kept constant even if the environmental temperature changes. It is therefore possible to obtain a stable peak retention time.
• Cooling function is included as standard
  The oven can regulate the temperature from ambient – 15℃ (Lower limit 4℃) up to ambient + 50℃ (upper limit 65℃).

The optics of the Primaide 1410 UV detector allows data collection with lower noise and drift. The lamp and flow cell are situated in a front-access layout and are easily replaceable.

• Low noise for high-sensitivity detection
  The Primaide 1410 UV detector achieves a noise level of no more than 0.5 × 10 ^-5 AU, greatly enhancing trace component analysis.
• High response and low noise for rapid analysis
  Low-noise measurements in 0.05 seconds.
  High-sensitivity detection rapidly yielding sharp and accurate peaks.
• Stable, without influence from ambient temperature changes
  Since local environments may vary considerably, the optics of the UV detector is well designed to avoid the effects of daily and seasonal temperature changes, ensuring stable and reproducible results.
• Wavelength measurement in the UV region with a built-in Hg lamp
  Use the 254-nm emission line of the mounted Hg lamp to test the accuracy of wavelength measurement in the UV region, which is the most utilized region in liquid chromatography. This test is conducted using 3 wavelengths from the Hg and D₂ lamps, enabling highly reliable analyses across a broad wavelength range.

The Primaide 1435 Diode Array Detector is as sensitive as a UV detector. The DAD system has many functions, including spectrum collection, chromatography using multiple wavelengths, a peak purity test, a spectral library, and more.

• High sensitivity
  Novel optics and improved software reduce noise considerably (0.5 × 10^-5 AU or less). The reputation, "DADs are less sensitive," is now obsolete.
• Minimizing the influence of temperature changes in the measurement cell
  The light source is stabilized via a large lamp housing. The spectrometer and housing are separated and thermally insulated. By cooling the lamp housing separately, the influence of ambient temperature is minimized. The new design incorporates temperature sensors as well as an associated variable rotation speed cooling fan, which largely eliminate temperature changes inside the instrument. This reduces noise and enhances stability, allowing for stable analysis day after day.
• High resolution and accurate spectra
  High wavelength resolution (0.78 nm/data point) is achieved with an array of 1,024 photo diodes. Uniform resolution is maintained throughout a wide range of wavelengths from the UV to VIS, thanks to the diffraction optics, which features a grating.
• Digital noise reduction in rapid analysis
  Data with a low level of noise are obtained in a very short sampling period (0.05 seconds). Noise-free, sharp peaks are obtained during rapid analysis.
• Automatic wavelength calibration
  A built-in Hg lamp is utilized in the automatic wavelength accuracy test for the commonly-used UV region.

Example of Diode Array Detector Data (acquired using ChromAssist® Data Station)
Please note: When purchasing the 1435 Diode Array Detector, the ChromAssist® Data Station must be purchased at the same time.

The Primaide 1440 FL detector has a wide range of wavelength setting and high sensitivity. The front access layout makes easy maintenance possible as same as the Primaide 1410 UV detector.

• Increased sensitivity with optical system that keeps light loss to a minimum
  The Primaide 1440 FL Detector is a bright optical system which has attained a S/N greater than 4000 versus a Raman peak of water (Background method)
• Selectable slit
  The slit of the emission side monochromator is selectable from 15nm and 30 nm. This allows analysis with a higher sensitivity.
• Automatic wavelength calibration
  Wavelength calibration is automated by the built-in Hg (mercury) lamp.
• Front panel access for easy maintenance
  User maintenance ( such as positional adjustment of Xe lamp, flow cell replacement, etc.) is facilitated through front panel access to the instrument.

The organizer can accept the simultaneous mounting of the following solvent bottles.

The organizer, which is also a power supply module, provides power to one pump, one autosampler, one column oven , one detector (one UV detector, or one Diode array detector), and one IFC board.

Applications

Introducing the measurement example using liquid chromatograph (HPLC) in various different fields.

HPLC Basic Course

The basics of HPLC, from "Principles and system configuration" to "Feature of various detection methods."

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