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HPLC for today and tomorrow
Two performance capabilities supporting data reliability: the excellent reproducibility made possible by the pump and autosampler and the excellent stability of the column oven and detector.
Beyond the simplicity of operation and ease of use, a critical requirement for HPLC is ease of maintenance.
The Hitachi reputation for instrument robustness and reliability continues with the Chromaster, which is made using stronger materials and is manufactured with Hitachi's strict quality control standards.
Pressure 5110: 40 MPa / 5160: 60 MPa
|Operating flow late range||0.001 to 9.999 mL/min||0.001 to 5.000 mL/min|
|Maximum operating pressure||40 MPa (0.001 to 5.000 mL/min)
20 MPa (5.001 to 9.999 mL/min)
|60 MPa (0.001 to 2.500 mL/min)
30 MPa (2.501 to 5.000 mL/min)
|Dimensions and weight||340(W) × 440(D) × 140(H) mm, Approx.16 kg|
|Number of mixed solvents*1||Up to 4|
5260: Withstand pressure 60 MPa, Loop injection method
5280: Withstand pressure 60 MPa, Direct injection method
|Sample injection system||Loop injection method (Cut injection, All volume injection, Full loop injection method)||Direct injection method|
|Withstand pressure||60 MPa||60 MPa|
|Washing function||Equipped with built-in washing pump
Two-solvent washing function
|Wash solvent delivery by a syringe|
|Sample rack temperature control||1 to 45 °C||1 to 35 °C|
|(Temperature setting range)||Capable of cooling and heating||Cooling only|
|Sample capacity||Standard||120 × 1.5 mL||200 × 1.5 mL|
|Optional||72 × 4 mL||128 × 4 mL|
5310: Horizontal type
6310: Vertical type
|Temperature setting range||1 to 85 °C (1 °C step)||4 to 90 °C (1 °C step)|
|Temperature control range||[Ambient temperature -15 °C] to [Ambient temperature +60 °C] and range of the temperature setting||[Ambient temperature -15 °C] to [Ambient temperature +75 °C] and within temperature setting range|
|Column capacity||300 mm × 3 (Maximum)|
|Built-in 3-liter water tank||-||Available|
|Dimensions and weight||410(W) × 440(D) × 140(H) mm, Approx. 13 kg||165(W) × 515(D) × 689(H) mm (Legs are not included), Approx. 25 kg|
5410: UV Detector
5420: UV-Vis Detector
5430: Diode Array Detector
5440: Fluorescence Detector
5450: RI Detector
5610: MS Detector
|Item||5410 UV Detector||5420 UV-Vis Detector||5430 Diode Array Detector|
|Optical system||Double-beam ratio photometric system||Single-beam ratio photometric system|
|Wavelength range||190 to 600 nm||190 to 900 nm||190 to 900 nm|
|Flow cell||Optical path length 10 mmm|
|Simultaneous measurement||Two-wavelength*||spectrum acquisition|
|Dimensions and weight||340(W) × 440(D) × 140(H) mm, Approx. 14kg|
|Item||5440 Fluorescence Detector|
|Light source||Xe lamp, Hg lamp for checking wavelength|
|Wavelength range||Ex: 200 to 850 nm
Em: 250 to 900 nm (Change photomultiplier at 731 nm or more)
|Flow cell||Irradiation volume 12 µL|
|Dimensions and weight||340 (W) × 440 (D) × 280 (H) mm, Approx. 25kg|
|Item||5450 RI Detector|
|Refractive index range||1 to 1.75|
|Temperature control range||OFF, and 30 to 50 °C|
|Dimensions and weight||340 (W) × 440 (D) × 140 (H) mm, excluding projections, Approx. 13 kg|
|Item||5610 MS Detector|
|Measurement mass range (m/z)||20 to 1,000|
|Ion source||Electrospray ionization (ESI)|
|Dimensions and weight||440 (W) × 610(D) × 430 (H) mm, Approx. 51 kg|
|N2 gas usage||Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa|
|Output power||DC24 V, 450 W
Supplies power to one pump, one autosampler, one detector (UV detector, UV-Vis detector, Diode array detector, or RI detector), and one interface control board
|Bottle capacity and space||1.0 L bottle × 6 and 500 mL bottle × 3(Maximum), 314(W) × 280.8(D) mm|
|Dimensions and weight||340 (W) × 420（D) × 200（H) mm, Approx. 9 kg|
The 60 MPa system, which can be used with columns containing 2.0 µm or smaller particles, and core-shell columns, is capable of conventional HPLC analysis and also ensures improved resolution performance and shorter analysis time.
|Column||LaChrom II C18 (5 µm) 4.6 mm I.D. × 150mmL
LaChromUltra II C18 (1.9 µm) 2.0 mm I.D. × 50 mmL
|Column temperature||40 °C|
|Mobile phase||A: H2O, B: CH3CN|
|Gradient mode||High Frequency Mode|
The following options are used with UHPLC column
Even when highly adsorbing Chlorhexidine is analyzed, the carry-over is extremely low.
Most optional accessories are internally mounted to reduce HPLC system height. The handle located on the front side of the organizer moves vertically for easy access to solvent bottles.
Module operations and the replacement of consumable and maintenance parts can be performed from the front side.
With attention to detail on the housing of tubes and wires, the system keeps tubes from getting tangled up, ensures the ease of replacement, and provides adequate seismic stability.
Hitachi column management system can manage the Log information on analytical columns and guard-columns from any manufacturer.
Log information can be written and read through a connector mounted on the column oven or USB port in the computer. ID Tags can be used repeatedly*.
Automatic system Wakeup and Sleep from GUI
The automation of system stand-by can reduce the amount of time required to make preparations for an analyses run.
Here are some latest applications measured by Chromaster.
Column switching valves enable automation of the pretreatment such as biological sample enrichment, removing impurities and so on.
And 2-wavelength measurement by real-time wavelength switching can be applied to the dissolution of resolvents and impurities in drug products.
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.
Introducing the measurement example using liquid chromatograph (HPLC) in various different fields.
The basics of HPLC, from "Principles and system configuration" to "Feature of various detection methods."
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