Analysis of Steroid Drugs
Drugs that contain a steroid skeleton include Prednisolone, Betamethasone, Dexamethasone, and Hydrocortisone, and each possesses a different duration of action and strength.
Steroid drugs providing powerful anti-inflammatory and immune suppression actions are used in the treatment of various illnesses, including allergy and inflammation ailments.
However, when administered continuously, steroid drugs can potentially trigger a variety of side-effects, such as obesity, high blood pressure, and diabetes. In view of this fact, when using steroids, treatment methods are needed that minimize side-effects through the proper adjustment of dosage, as well as methods of administration in a manner that is appropriate for a given disease.
The text below describes analyses that were performed on two types of off-the-shelf ointments.
Analysis of steroid drugs
Samples
Commercially available ointments A and B
Sample preparation
Standard samples
System configuration
5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System
Conditions
| Column | HITACHI LaChrom C18 (5 µm) (4.6 mmI.D. x 150 mm) |
|---|---|
| Mobile phase | H2O/MeOH = 40/60 (V/V) |
| Flow rate | 1.0 mL/min |
| Column Temperature | 40°C |
| Detection | 254 nm |
| Injection vol. | 10 µL |
Results of standard sample assaying
Linearity
(Range of calibration: 0.1 - 20 µg/mL)
Conditions
| Column | HITACHI LaChrom C18 (5 µm) (4.6 mmI.D. x 150 mm) |
|---|---|
| Mobile phase | H2O/MeOH = 40/60 (V/V) |
| Flow rate | 1.0 mL/min |
| Column Temperature | 40°C |
| Detection | 254 nm |
| Injection vol. | 10 µL |
Results of analysis
As a result of analyzing two off-the-shelf ointments, hydrocortisone acetate was detected in Ointment A, and Prednisolone in Ointment B.
NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.
In order to read a PDF file, you need to have Adobe® Reader®
