Simultaneous Analysis of Four Agricultural Chemical Components
Iprodione, asulam, thiophanate-methyl, and siduron are four agricultural chemicals that are designated as water quality management target items. Simultaneous analysis of these chemicals typically requires the use of LC/MS. The accuracy of this analysis must be within 20% of the coefficient of variation, and is determined by analyzing samples that are 1/100 of the concentration of target values (0.003 mg/L or 0.002 mg/L for asulam). If this sample is concentrated by a factor of 500, the resulting concentration (1.5 mg/L or 1.0 mg/L for asulam) is within the measurement capability of a high-sensitivity DAD system (See Technical Data No.187).
The text below describes examples of analyses performed.
Analysis of four agricultural chemical components
Component and Target value
| Name of chemical | Component | Molecular formula | Application | Target value [mg/L] |
|---|---|---|---|---|
| Iprodione | 3-(3,5-dichlorophenyl)-N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide | C13H13Cl2N3O3 | Germicide | 0.3 |
| Asulam | methyl sulfanilycarbamate | C8H10N2O4S | Herbicide | 0.2 |
| Thiophanate-methyl | dimethyl 4, 4' -(o-phenylene) bis (3-thioallophanate) | C12H14N4O4S2 | Germicide | 0.3 |
| Siduron | 1-(2-methylcyclohexyl)-3-phenylurea | C14H20N2O | Herbicide | 0.3 |
Sample preparation
Standard solution: Prepared with acetonitrile so that the concentration will be 0.2 - 20 mg/L.
Solid phase extraction procedure
*
NOBIAS RP-SG1
A reverse-phase polymer-based solid-phase packed column.
Example of analysis of a standard sample
Sample concentration
1.0 mg/L per component
System configuration
5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System
Conditions
| Column | HITACHI LaChrom C18 (3 µm) (4.6 mmI.D. x 150 mm) |
|---|---|
| Mobile phase | 50 mM potassium dihydrogen phosphate (pH 3.0)/ acetonitrile = 45/55 |
| Flow rate | 1.0 mL/min |
| Column Temperature | 40°C |
| Detection | 270 nm Asulam 230 nm thiophanate-methyl, siduron, iprodione |
| Injection vol. | 10 µL |
Linearity
At a 0.2 to 20 mg/L concentration, the sample produces a favorable straight line. [Asulum]R2: 0.9998,[Thiophanatemethyl]R2: 0.9999 [Siduron(1)] R2: 0.9999, [Siduron(2)], R2: 0.9999, [Iprodione] R2: 0.9999
NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.
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