Analysis of Anion Surfactants
Anion surfactants interfere with wastewater treatment processes due to their foaming properties. A standard analysis mixture consists of five sodium alkylbenzenesulfonates of varying alkyl chain length (C10-C14) at a concentration of 0.004 mg/L each, for a total surfactant concentration of 0.02 mg/L. The method includes a solid phase extraction step followed by HPLC analysis, and is described in Table 24 of the bulletin. The data describes the analysis of a five component mixture of sodium alkylbenzenesulfonates at a concentration of 0.004 mg/L each, concentrated 250-fold to a concentration of 1 mg/L in a pretreatment step.
Analysis of anion surfactants
Sample
Alkyl benzenesulfonate (the active ingredient of a synthetic detergent)
Solid phase extraction procedure
Solid phase extraction procedure
*1: NOBIAS RP-OD1
This is a reverse-phase polymer-based column, and it is a solid-phase packed column in which an octadecyl silyl group is linked to a hydrophilic methacrylate substrate.
System configuration
5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System
Conditions
| Column | HITACHI -Inertsil ODS-3 (3 µm) (4.6 mmI.D. x 150 mm) |
|---|---|
| Elute | 0.1 mol/L sodium perchlorate - (CH3CN : H2O = 65 : 35) |
| Flow rate | 0.6 mL/min |
| Column Temperature | 40°C |
| Detection | FL Ex 221 nm, Em 284 nm |
| Injection vol. | 20 µL |
Results of measurement of standard samples
C10BS: Sodium Decylbenzenesulfonate
C11BS: Sodium Undecylbenzenesulfonate
C12BS: Sodium Dodecylbenzenesulfonate
C13BS: Sodium Tridecylbenzenesulfonate
C14BS: Sodium Tetradecylbenzenesulfonate
The toluene used in the pretreatment for the analysis of a non-ionic surfactant, another test item, can affect the analysis of the anion surfactant.
An evaluation of elution conditions indicates that toluene is eluted between C12 and C13; therefore, high-precision analyses with any impact on quantitation are possible
Verification of separation from toluene
Conditions
| Column | HITACHI -Inertsil ODS-3 (3 µm) (4.6 mmI.D. x 150 mm) |
|---|---|
| Elute | 0.1 mol/L sodium perchlorate - (CH3CN : H2O = 65 : 35) |
| Flow rate | 0.6 mL/min |
| Column Temperature | 40°C |
| Detection | FL Ex 221 nm, Em 284 nm |
| Injection vol. | 20 µL |
NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.
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