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Hitachi

Hitachi High-Technologies GLOBAL

Iprodione, asulam, thiophanate-methyl, and siduron are four agricultural chemicals that are designated as water quality management target items. Simultaneous analysis of these chemicals typically requires the use of LC/MS. The accuracy of this analysis must be within 20% of the coefficient of variation, and is determined by analyzing samples that are 1/100 of the concentration of target values (0.003 mg/L or 0.002 mg/L for asulam). If this sample is concentrated by a factor of 500, the resulting concentration (1.5 mg/L or 1.0 mg/L for asulam) is within the measurement capability of a high-sensitivity DAD system (See Technical Data No.187).

The text below describes examples of analyses performed.

Analysis of four agricultural chemical components

Component and Target value

Name of chemical Component Molecular formula Application Target value
[mg/L]
Iprodione 3-(3,5-dichlorophenyl)-N-isopropyl-2,4-dioxoimidazolidine-1-carboxamide C13H13Cl2N3O3 Germicide 0.3
Asulam methyl sulfanilycarbamate C8H10N2O4S Herbicide 0.2
Thiophanate-methyl dimethyl 4, 4' -(o-phenylene) bis (3-thioallophanate) C12H14N4O4S2 Germicide 0.3
Siduron 1-(2-methylcyclohexyl)-3-phenylurea C14H20N2O Herbicide 0.3

Sample preparation

Standard solution: Prepared with acetonitrile so that the concentration will be 0.2 - 20 mg/L.

Solid phase extraction procedure

*
NOBIAS RP-SG1
A reverse-phase polymer-based solid-phase packed column.

Example of analysis of a standard sample

Sample concentration

1.0 mg/L per component

System configuration

5110 Pump
5210 AutoSampler
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System

Conditions

Column HITACHI LaChrom C18 (3 µm) (4.6 mmI.D. x 150 mm)
Mobile phase 50 mM potassium dihydrogen phosphate (pH 3.0)/ acetonitrile = 45/55
Flow rate 1.0 mL/min
Column Temperature 40°C
Detection 270 nm Asulam
230 nm thiophanate-methyl, siduron, iprodione
Injection vol. 10 µL

Linearity

At a 0.2 to 20 mg/L concentration, the sample produces a favorable straight line. [Asulum]R2: 0.9998,[Thiophanatemethyl]R2: 0.9999 [Siduron(1)] R2: 0.9999, [Siduron(2)], R2: 0.9999, [Iprodione] R2: 0.9999

NOTE:
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.

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