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Iprodione, asulam, thiophanate-methyl, and siduron are four agricultural chemicals that are designated as water quality management target items. Simultaneous analysis of these chemicals typically requires the use of LC/MS. The accuracy of this analysis must be within 20% of the coefficient of variation, and is determined by analyzing samples that are 1/100 of the concentration of target values (0.003 mg/L or 0.002 mg/L for asulam). If this sample is concentrated by a factor of 500, the resulting concentration (1.5 mg/L or 1.0 mg/L for asulam) is within the measurement capability of a high-sensitivity DAD system (See Technical Data No.187).
The text below describes examples of analyses performed.
Component and Target value
|Name of chemical||Component||Molecular formula||Application||Target value
|Thiophanate-methyl||dimethyl 4, 4' -(o-phenylene) bis (3-thioallophanate)||C12H14N4O4S2||Germicide||0.3|
Standard solution: Prepared with acetonitrile so that the concentration will be 0.2 - 20 mg/L.
Solid phase extraction procedure
Example of analysis of a standard sample
1.0 mg/L per component
5310 Column Oven
5420 UV-VIS Detector
Empower2 Data Processing System
|Column||HITACHI LaChrom C18 (3 µm) (4.6 mmI.D. x 150 mm)|
|Mobile phase||50 mM potassium dihydrogen phosphate (pH 3.0)/ acetonitrile = 45/55|
|Flow rate||1.0 mL/min|
|Detection||270 nm Asulam
230 nm thiophanate-methyl, siduron, iprodione
|Injection vol.||10 µL|
At a 0.2 to 20 mg/L concentration, the sample produces a favorable straight line. [Asulum]R2: 0.9998,[Thiophanatemethyl]R2: 0.9999 [Siduron(1)] R2: 0.9999, [Siduron(2)], R2: 0.9999, [Iprodione] R2: 0.9999
These data are an example of measurement; the individual values cannot be guaranteed.
The system is for research use only, and is not intended for any animal or human therapeutic or diagnostic use.