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Three Dimensional Structure of the HeLa Cell

© Katsuji Ito, Manabu Shirai, Mami Konomi, Eiko Nakazawa (Hitachi High-Tech Corporation)
© Sumire Inaga (Tottori University, Faculty of Medicine)

Three dimensionally reconstructed images of a cultured HeLa cell stained with platinum blue are presented; the images are composed of cross-section images serially acquired with 10 nm steps using an FIB-SEM.
Morphologies of chromosomes or cytoplasmic processes and their dispersion states in the cell are clearly visualized. It is expected that higher-order structure of unknown chromosomes will be elucidated by this technique.

1st Prize. At 73th photo contest hosted by the Japanese Society of Microscopy in 2017.


Specimen : Cultured HeLa cell

Instrument: Focused Ion & Electron Beam System nanoDUE'T NB5000

Magnification : X 4,000

Accelerating voltage : 3 kV (BSE)

*This work was presented at the "photo contest" hosted by the Japanese Society of Microscopy.

*Reproduction or republication without permission prohibited.

*"nanoart" is registered trademark of Hitachi High-Tech Corporation in Japan.

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